A multi‐omics approach to overeating and inactivity‐induced muscle atrophy in db/db mice

Autor: Takuro Okamura, Masahide Hamaguchi, Genki Kobayashi, Takahiro Ichikawa, Yuka Hasegawa, Tomoki Miyoshi, Takafumi Senmaru, Naoko Nakanishi, Ryoichi Sasano, Michiaki Fukui
Jazyk: angličtina
Rok vydání: 2024
Předmět:
Zdroj: Journal of Cachexia, Sarcopenia and Muscle, Vol 15, Iss 5, Pp 2030-2045 (2024)
Druh dokumentu: article
ISSN: 2190-6009
2190-5991
11781432
DOI: 10.1002/jcsm.13550
Popis: Abstract Background Overeating and inactivity are associated with type 2 diabetes. This study aimed to investigate its pathological basis using integrated omics and db/db/mice, a model representing this condition. Methods The study involved housing 8‐week‐old db/m and db/db mice for 8 weeks. Various analyses were conducted, including gene expression in skeletal muscle and small intestine using next‐generation sequencing; cytokine arrays of serum; assessment of metabolites in skeletal muscle, stool, and serum; and analysis of the gut microbiota. Histone modifications in small intestinal epithelial cells were profiled using CUT&Tag. Results Compared with db/m mice, db/db mice had 22.4% lower grip strength and approximately five times the visceral fat weight (P
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