| Autor: |
Gurgiane Rodrigues Gurgel CAVALCANTE, Mariana Cabral MORENO, Flavia Queiroz PIRIH, Vanessa de Paula SOARES, Éricka Janine Dantas da SILVEIRA, José Sandro Pereira da SILVA, Hallissa Simplício Gomes PEREIRA, Katherine Pennington KLEIN, Maria Luiza Diniz de Sousa LOPES, Aurigena Antunes de ARAUJO, Agnes Andrade MARTINS, Ruthinéia Diógenes Alves Uchôa LINS |
| Jazyk: |
angličtina |
| Rok vydání: |
2024 |
| Předmět: |
|
| Zdroj: |
Brazilian Oral Research, Vol 38 (2024) |
| Druh dokumentu: |
article |
| ISSN: |
1807-3107 |
| DOI: |
10.1590/1807-3107bor-2024.vol38.0131 |
| Popis: |
Abstract The aim of this study was to investigate the effect of thermogenic supplementation on the bone tissue of rats subjected to orthodontic movement. A total of 38 male Wistar rats underwent orthodontic movement of the left permanent maxillary first molars for 21 days. The rats were assigned to three groups: Control group: water; Thermogenic 1: C4 Beta Pump thermogenic; or Thermogenic 2: PRE-HD/Pre-workout. Micro-computed tomography (micro-CT) was used to investigate the dynamic changes in the microstructure of alveolar bone during orthodontic tooth movement in rats. Histopathologic analysis was performed by hematoxylin and eosin (H&E) staining, whereas tartrate-resistant acid phosphatase (TRAP) was employed for osteoclast count. Maxillary tissue was collected and evaluated by immunohistochemistry for receptor activator of NF-κB (RANK), receptor activator of NF-κB ligand (RANKL), and osteoprotegerin (OPG). The Thermogenic 2 group exhibited a significantly lower percentage of bone volume fraction (BV/TV) (68.21% ± 17.70%) compared to the control (86.84% + 12.91%) and Thermogenic 1 groups (86.84% + 15.94%) (p < 0.05). The control group had a significantly higher mean orthodontic movement in the mesial direction (0.2143 mm + 0.1513 mm) than the Thermogenic 2 group (0.0420 mm + 0.05215 mm) (p < 0.05). The Thermogenic 2 and Thermogenic 1 groups showed a stronger immunostaining for RANKL when compared to the control group (p < 0.05). The supplementation used in the Thermogenic 2 group (PRE-HD/Pre-workout) induced alveolar bone loss in rats subjected to orthodontic movement, which can be related to the regulation of the RANK/RANKL/OPG signaling pathway. This suggests the influence of thermogenic supplements on bone metabolism seems to depend on their composition. |
| Databáze: |
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