| Autor: |
Marco Antonio Hernández-Luna, Ricardo Díaz de León-Ortega, Daniel Dimitri Hernández-Cueto, Ricardo Gaxiola-Centeno, Raúl Castro-Luna, Leonel Martínez-Cristóbal, Sara Huerta-Yépez, Rosendo Luria-Pérez |
| Jazyk: |
English<br />Spanish; Castilian |
| Rok vydání: |
2016 |
| Předmět: |
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| Zdroj: |
Boletín Médico del Hospital Infantil de México, Vol 73, Iss 6, Pp 388-396 (2016) |
| Druh dokumentu: |
article |
| ISSN: |
1665-1146 |
| DOI: |
10.1016/j.bmhimx.2016.10.002 |
| Popis: |
Background: Tumor cell resistance to chemotherapy agents is one of the main problems in the eradication of different neoplasias. One of the mechanisms of this process is the overexpression of anti-apoptotic proteins such as Bcl-2 and Bcl-XL; blocking the activity of these proteins may contribute to the sensitization of tumor cells and allow the adequate effects of chemotherapeutic drugs. Methods and results: This study adressed the transfection of prostate cancer cells (PC3) with a plasmid encoding a recombinant protein with an antagonist peptide from the BH3 region of the Bax protein fused to the GFP reporter protein (BaxGFP). This protein induced apoptosis of these tumor cells; further, selective transport of this plasmid to the tumor cell with Salmonella enterica serovar Typhimurium (strain SL3261), a live-attenuated bacterial vector, can induce sensitization of the tumor cell to the action of drugs such as cisplatin, through a process known as bactofection. Conclusions: These results suggest that Salmonella enterica can be used as a carrier vector of nucleotide sequences encoding heterologous molecules used in antitumor therapy. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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