Effects of metronidazole on the fecal microbiome and metabolome in healthy dogs

Autor: Rachel Pilla, Frederic P. Gaschen, James W. Barr, Erin Olson, Julia Honneffer, Blake C. Guard, Amanda B. Blake, Dean Villanueva, Mohammad R. Khattab, Mustafa K. AlShawaqfeh, Jonathan A. Lidbury, Jörg M. Steiner, Jan S. Suchodolski
Jazyk: angličtina
Rok vydání: 2020
Předmět:
Zdroj: Journal of Veterinary Internal Medicine, Vol 34, Iss 5, Pp 1853-1866 (2020)
Druh dokumentu: article
ISSN: 1939-1676
0891-6640
DOI: 10.1111/jvim.15871
Popis: Abstract Background Metronidazole has a substantial impact on the gut microbiome. However, the recovery of the microbiome after discontinuation of administration, and the metabolic consequences of such alterations have not been investigated to date. Objectives To describe the impact of 14‐day metronidazole administration, alone or in combination with a hydrolyzed protein diet, on fecal microbiome, metabolome, bile acids (BAs), and lactate production, and on serum metabolome in healthy dogs. Animals Twenty‐four healthy pet dogs. Methods Prospective, nonrandomized controlled study. Dogs fed various commercial diets were divided in 3 groups: control group (no intervention, G1); group receiving hydrolyzed protein diet, followed by metronidazole administration (G2); and group receiving metronidazole only (G3). Microbiome composition was evaluated with sequencing of 16S rRNA genes and quantitative polymerase chain reaction (qPCR)‐based dysbiosis index. Untargeted metabolomics analysis of fecal and serum samples was performed, followed by targeted assays for fecal BAs and lactate. Results No changes were observed in G1, or G2 during diet change. Metronidazole significantly changed microbiome composition in G2 and G3, including decreases in richness (P
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