| Autor: |
Kayode Komolafe, Afolabi C. Akinmoladun, Titilope R. Komolafe, Mary T. Olaleye, Akintunde A. Akindahunsi, Joao B. T. Rocha |
| Jazyk: |
angličtina |
| Rok vydání: |
2017 |
| Předmět: |
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| Zdroj: |
Clinical Phytoscience, Vol 3, Iss 1, Pp 1-11 (2017) |
| Druh dokumentu: |
article |
| ISSN: |
2199-1197 |
| DOI: |
10.1186/s40816-017-0057-4 |
| Popis: |
Abstract Background Parkia biglobosa leaf has popular ethnomedicinal use in tropical Africa. However, little is known about its molecular biological effects. This study sought to investigate the in vitro antioxidant activity, angiotensin-I-converting enzyme (ACE) inhibition and effects of aqueous-methanolic extract of P. biglobosa leaf (PBE) on mitochondrial membrane potential and reactive oxygen species (ROS) generation. Methods Antioxidant activity was determined by extract’s DPPH. (1,1-diphenyl-2-picrylhydrazyl radical), ABTS.+ [2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt radical cation] scavenging ability, reducing property and propensity to inhibit lipid peroxidation induced by prooxidants (FeSO4/sodium nitroprusside, SNP) in isolated rat tissue preparations. Determination of angiotensin-converting enzyme (ACE) inhibition was based on the hydrolysis of N-hippuryl-His-Leu hydrate (HHL) by the enzyme. Subsequently, the effects of PBE on toxicant-induced mitochondrial ROS formation and basal membrane potential (∆Ψm) were determined by 2′, 7′-dichlorodihydrofluorescin (DCFH) oxidation and safranine fluorescence respectively. Results PBE significantly reduced ferric ions (P |
| Databáze: |
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| Externí odkaz: |
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