Protocol for collection and separation of bone marrow mononuclear cells in Chlorocebus aethiops
| Autor: | Érika Branco, Klena Sarges Marruaz Silva, Maria de Fátima Assis, Edivaldo Herculano Corrêa de Oliveira, Ana Rita de Lima, Ana Cleide Guimbal de Aquino, Rosa Cabral, Maria Angelica Miglino |
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| Jazyk: | English<br />Portuguese |
| Rok vydání: | 2016 |
| Předmět: | |
| Zdroj: | Pesquisa Veterinária Brasileira, Vol 36, Iss 2, Pp 119-122 (2016) |
| Druh dokumentu: | article |
| ISSN: | 1678-5150 0100-736X |
| DOI: | 10.1590/S0100-736X2016000200009 |
| Popis: | Abstract: Chlorocebus aethiops is a species of non-human primate frequently used in biomedical research. Some research involves this species as an experimental model for various diseases and possible treatment with stem cells. The bone marrow is one of the main sources of these cells and provides easy access. The aim of this study was to standardize the protocol of collection and separation of bone marrow in C. aethiops. Ten animals were submitted to puncture of bone marrow with access to the iliac crest and cell separation by density gradient. The bone marrow of C. aethiops had an average of 97% viability. From the results achieved, we can conclude that C. aethiops is an excellent model to obtain and isolate mononuclear cells from bone marrow, fostering several studies in the field of cell therapy. |
| Databáze: | Directory of Open Access Journals |
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