Lofty frequency and reproducible plant regeneration from mature nodal explants of ‘‘Egusi’’melon (Citrullus colocynthis L.)
| Autor: | Prabhu Thangavel, Sisubalan Natarajan, Velmurugam Shanmugam, Sheik Sulaiman, Ravikumar Ramasamy |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: | |
| Zdroj: | BioTechnologia, Vol 100, Iss 3, Pp 263-272 (2019) |
| Druh dokumentu: | article |
| ISSN: | 0860-7796 2353-9461 |
| DOI: | 10.5114/bta.2019.87585 |
| Popis: | The present study attempts to explain a mass culture protocol for large scale multiple shoot bud regeneration from nodal explants of Citrullus colocynthis L. The highest shoot bud induction (94.9%) without attaining callus was observed in Murashige and Skoog’s (MS) medium supplemented with benzyl amino purine (BA)in which the combination of benzyl amino purine BAP (1.0 mg/l), kinetin (0.5 mg/l), gibberellic acid (1.5 mg/l) produced more shoots/explants. This study strongly suggests that the pre-eminence of BA and other cytokinins in combination with GA3 was found to be essential for a swift multiple shoot bud induction as well as an enhanced rate of shoot proliferation in C. colocynthis. A full-strength MS medium, supplemented with indole 3 butyric acid IBA (1.5 mg/l) combined with BA (1.5 mg/l), had the maximum rooting response and the regenerated plantlets were successfully established in the field with an 80% survival rate. |
| Databáze: | Directory of Open Access Journals |
| Externí odkaz: |
|