A Modified Method for Measuring Nτ-Methylhistidine in Chiken Feed and Excreta
| Autor: | Yahya Z. Eid, Akira Ohtsuka, Kunioki Hayashi |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2001 |
| Předmět: | |
| Zdroj: | The Journal of Poultry Science, Vol 38, Iss 4, Pp 354-357 (2001) |
| Druh dokumentu: | article |
| ISSN: | 1346-7395 1349-0486 |
| DOI: | 10.2141/jpsa.38.354 |
| Popis: | Analysis of Nτ-methylhistidine is intricate and time consuming. In the present study, a modified technique using high-performance liquid chromatography, which utilizes a reversed-phase separation with ion pairing and post column fluorescence derivatization for the analysis of Nτ-methylhistidine in chicken feed and excreta is described. Two ml of the hydrolyzed sample was applied directly into the ion-exchange resin column without evaporation of HCl to roughly separate Nτ-methylhistidine from acidic and neutral amino acids. The Nτ-methylhistidine fraction was eluted with 1M pyridine and then evaporated, and the residue was dissolved in the mobile phase and subjected to high-performance liquid chromatography. The present method shortened the time needed to complete the assay by about 30min per sample and improved the separation of Nτ-methylhistidine. |
| Databáze: | Directory of Open Access Journals |
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