| Autor: |
Anna Kukkola, Stefan Krause, Yasmin Yonan, Liam Kelleher, Uwe Schneidewind, Gregory H. Sambrook Smith, Holly Nel, Iseult Lynch |
| Jazyk: |
angličtina |
| Rok vydání: |
2023 |
| Předmět: |
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| Zdroj: |
MethodsX, Vol 10, Iss , Pp 102053- (2023) |
| Druh dokumentu: |
article |
| ISSN: |
2215-0161 |
| DOI: |
10.1016/j.mex.2023.102053 |
| Popis: |
We present here a technique for setting up detection limits on any fluorescent microscope in conjunction with the fluorophore Nile Red for microplastic identification. Our method also describes a rigorous morphology-specific identification key for microplastics to reduce subjectivity between researchers. The detection limits were established for nine common polymer types and five natural substrates which could result in false-positive signals when using Nile Red for microplastic identification. This method was then applied to real freshwater samples and identified particles were validated with micro-FTIR or Raman spectroscopy. This approach may reduce subjectivity in microplastic identification and counting and enhances transparency, repeatability and harmonization within microplastic research community. • Instructions for calibration of detection limits for microplastics on fluorescence microscope systems described • Microplastic identification key developed and tested to reduce false positive detection • Lower subjectivity for microplastic identification obtained using the detection limits & identification key |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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