| Autor: |
Shanmugam Murugesan, Ramkrishna Mahapatra |
| Jazyk: |
angličtina |
| Rok vydání: |
2022 |
| Předmět: |
|
| Zdroj: |
Brazilian Archives of Biology and Technology, Vol 65 (2022) |
| Druh dokumentu: |
article |
| ISSN: |
1678-4324 |
| DOI: |
10.1590/1678-4324-2022210056 |
| Popis: |
Abstract The aim of this study was to assess permeable cryoprotectants and dextran during broiler breeder lines semen cryopreservation in two separate experiments. Semen of PB-2 broiler breeder line was cryopreserved with 8% Ethylene Glycol (EG) or 6% dimethylformamide (DMF) in Beltsville Poultry Semen Extender (BPSE). In another experiment semen of Control broiler (CB) breeder line was cryopreserved with 8% EG, 8.2% dextran 10 kDa + 9% dimethylacetamide (DMA) or 8.2% dextran 20 kDa + 9% dimethylacetamide (DMA) in Sasaki diluent (SD). Semen cryopreserved in 0.5 ml plastic straw was thawed either at 5°C for 100 sec or 37°C for 30 sec. Semen was assessed for motility, live sperm, abnormal sperm and acrosome intact sperm. Post-thaw semen was inseminated, eggs were collected and incubated for obtaining fertility and hatchability data. The cryopreserved samples had significantly (p |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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