| Autor: |
Alexandre Calon, Elisa Espinet, Sergio Palomo-Ponce, Daniele Tauriello, Mar Iglesias, María Céspedes, Marta Sevillano, Cristina Nadal, Peter Jung, Xiang Zhang, Daniel Byrom, Antoni Riera, David Rossell, Ramón Mangues, Joan Massague, Elena Sancho, Eduard Batlle |
| Jazyk: |
angličtina |
| Rok vydání: |
2014 |
| Předmět: |
|
| Zdroj: |
Bio-Protocol, Vol 4, Iss 9 (2014) |
| Druh dokumentu: |
article |
| ISSN: |
2331-8325 |
| DOI: |
10.21769/BioProtoc.1119 |
| Popis: |
Metastasis depends on a gene program expressed by the tumor microenvironment upon TGF-beta stimulation. CRC (Colorectal cancer) cell lines did not induce robust stromal TGF-beta responses when injected into nude mice as shown by lack of p-SMAD2 accumulation in tumor-associated stromal cells. To enforce high TGF-beta signaling in xenografts, we engineered CRC cell lines to secrete active TGF-beta. Subcutaneous tumors obtained from HT29-M6TGF-β, KM12L4aTGF-β cells and SW48TGF-β cells contained abundant p-SMAD2+ stromal cells. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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