| Autor: |
Gabriella Boisen, Susanne Brogårdh-Roth, Jessica Neilands, Alex Mira, Miguel Carda-Diéguez, Julia R. Davies |
| Jazyk: |
angličtina |
| Rok vydání: |
2024 |
| Předmět: |
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| Zdroj: |
Frontiers in Oral Health, Vol 5 (2024) |
| Druh dokumentu: |
article |
| ISSN: |
2673-4842 |
| DOI: |
10.3389/froh.2024.1475361 |
| Popis: |
IntroductionDuring development of dental caries, oral biofilms undergo changes in microbial composition and phenotypical traits. The aim of this study was to compare the acid tolerance (AT) of plaque from two groups of children: one with severe caries (CA) and one with no caries experience (CF) and to correlate this to the microbial composition and metabolic profile of the biofilms.MethodsDental plaque samples from 20 children (2–5 years) in each group were studied. The AT was analyzed by viability assessment after exposure to an acid challenge (pH 3.5), using LIVE/DEAD® BacLight™ stain and confocal microscopy. Levels of acid tolerance (AT) were evaluated using a scoring system ranging from 1 (no/low AT), to 5 (high/all AT). Metabolic profiles were investigated following a 20 mM glucose pulse for one hour through Nuclear Magnetic Resonance (NMR). Microbial composition was characterized by 16S rRNA Illumina sequencing.ResultsThe mean AT score of the CA group (4.1) was significantly higher than that of the CF group (2.6, p |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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