In Vitro Cytotoxicity Assessment of an Orthodontic Composite Containing Titanium-dioxide Nano-particles
| Autor: | Farzin Heravi, Mohammad Ramezani, Maryam Poosti, Mohsen Hosseini, Arezoo Shajiei, Farzaneh Ahrari |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: | |
| Zdroj: | Journal of Dental Research, Dental Clinics, Dental Prospects, Vol 7, Iss 4, Pp 192-198 (2013) |
| Druh dokumentu: | article |
| ISSN: | 2008-210X 2008-2118 |
| DOI: | 10.5681/joddd.2013.031 |
| Popis: | Background and aims. Incorporation of nano-particles to orthodontic bonding systems has been considered to prevent enamel demineralization around appliances. This study investigated cytotoxicity of Transbond XT adhesive containing 1 wt% titanium dioxide (TiO2) nano-particles. Materials and methods. Ten composite disks were prepared from each of the conventional and TiO2-containg composites and aged for 1, 3, 5, 7 and 14 days in Dulbecco’s Modified Eagle’s Medium (DMEM). The extracts were obtained and exposed to culture media of human gingival fibroblasts (HGF) and mouse L929 fibroblasts. Cell viability was measured using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Results. Both adhesives were moderately toxic for HGF cells on the first day of the experiment, but the TiO2-containing adhesive produced significantly lower toxicity than the pure adhesive (P0.05). There was a significant reduction in cell toxicity with increasing pre-incubation time (P |
| Databáze: | Directory of Open Access Journals |
| Externí odkaz: |
|