Mesothelial Cell Adherence to Vascular Prostheses and Their Subsequent Growth In Vitro

Autor: Apollo Pronk, Arthur A.G.M. Hoynck Van Papendrecht, Piet Leguit, Henri A. Verbrugh, Roel P.A.J. Verkooyen, Theo J.M.V. Van Vroonhoven
Jazyk: angličtina
Rok vydání: 1994
Předmět:
Zdroj: Cell Transplantation, Vol 3 (1994)
Druh dokumentu: article
ISSN: 0963-6897
1555-3892
09636897
DOI: 10.1177/096368979400300107
Popis: Cell seeding may decrease the thrombogenicity of implanted vascular grafts, but its application is hampered by the limited availability of autologous endothelial cells. Human peritoneal mesothelial cells have blood flow supporting qualities and are readily available. This study investigated the adherence of mesothelial cells to vascular prostheses and their subsequent growth in vitro. Circular pieces of various vascular prosthetic materials were seeded with 51Chromium-labeled mesothelial and endothelial cells and left for either 5, 15, 30, 60, and 120 minutes. The unattached cells were removed and the degree of cell attachment was measured. The number of mesothelial cells to Dacron increased during the first 60 min up to 35.2 % of the seeded inoculum whereafter a plateau was reached. Scanning electron microscopy showed spreaded mesothelial cells adherent to the Dacron fibers. A significant increase in adherence was observed after preincubation of Dacron with 10 μg/mL fibronectin, but no improvement was found after preincubation with human serum albumin or gelatin. Mesothelial cells adhered better to Gelcoated than to Gelsealed or plain Dacron. The adherence of mesothelial cells to ePTFE (Teflon) was significantly poorer. No significant differences in adherence were found between mesothelial and endothelial cells. Mesothelial cell growth on Dacron resulted in a modest increase in the number of viable cells during 27 days, which implies biocompatibility of Dacron and mesothelial cells in vitro.
Databáze: Directory of Open Access Journals
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