Impact of a High-fat Diet on the Development of Chronic Inflammation in Heart of Wistar rats

Autor: Iliyan V. Dimitrov, Vassil I. Kamenov, Nikolay P. Boyadjiev, Katerina N. Georgieva, Anelia V. Bivolarska, Milena N. Draganova-Filipova, Penka A. Angelova-Hristova, Slavi Delchev, Elena Daskalova, Fanka Gerginska, Teodora R. Stankova, Vilian Gramatikov
Jazyk: angličtina
Rok vydání: 2019
Předmět:
Zdroj: Folia Medica, Vol 61, Iss 3, Pp 404-410 (2019)
Druh dokumentu: article
ISSN: 1314-2143
0204-8043
DOI: 10.3897/folmed.61.e39348
Popis: Introduction: Obesity is linked to the development of low-grade, chronic inflammation. Obesity-related inflammation appears to be a different type of inflammation, mainly due to excessive food intake and unusual homeostasis. It can be evaluated by measuring the concentration of pro- and anti-inflammatory marker molecules – C-reactive protein (CRP), serum amyloid-A (SAA) and interleukin-4.Aim: The aim of the present study is to evaluate the rate of the inflammatory process in heart, provoked by the consumption of a high-fat diet.Materials and methods: Sixty 8-week-old male Wistar rats were used in this experiment. The laboratory animals were fed orally with two different types of rodent food for 14 or 18 weeks – a high-fat diet (experimental groups) and standard rodent food (control groups). They all were kept under standard housing conditions. The levels of the pro- and anti-inflammatory markers in tissue homogenates from heart were analyzed using ELISA. Their expression in tissue samples was detected immunohistochemically by the biotin-streptavidin-peroxidase method. The total protein concentration was determined by the Lawry method.Results: CRP levels showed no significant differences when the control group was compared with the groups fed with a high-fat diet (p>0.05). The SAA levels detected were also insignificantly changed. Only the IL-4 tissue levels showed tendency to increase (p
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