Levan Production in Shake Flask and Fermenter Influence of Feeding Strategy on Levan Yield and Molecular Weight Distribution

Autor: Veerapandian Bhuvaneshwari, Ramiah Shanmugam Saravanan, Krishnan Srividhya, Sivaraman Subramaniyasharma, Sriariyanun Malinee, Venkatachalam Ponnusami
Jazyk: English<br />French
Rok vydání: 2023
Předmět:
Zdroj: E3S Web of Conferences, Vol 428, p 02005 (2023)
Druh dokumentu: article
ISSN: 2267-1242
DOI: 10.1051/e3sconf/202342802005
Popis: Effect of feeding strategy on levan production was studied in a shake flask and a 5 L lab scale fermenter. In a shake flask, levan specific substrate yield (YP/S) increased from 0.35 g levan/g sucrose to 0.48 g levan/g sucrose with repeated batch feeding. In a 3 L reactor, levan productivity of 7.18 g/L h was obtained with the fed-batch mode of fermentation. The gel permeation chromatography results indicate that higher initial sucrose concentration under the fed-batch mode operation resulted in the formation of low molecular weight fractions (4 and 10 kDa). Thus, fed-batch fermentation favors levan production resulting in higher yield and productivity and also affects the molecular weight distribution of the biopolymer.
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