| Autor: |
Nicole Di Lascio, Francesca Lenzarini, Cristina Barsanti, Francesco Stea, Claudia Kusmic, Francesco Faita |
| Jazyk: |
angličtina |
| Rok vydání: |
2015 |
| Předmět: |
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| Zdroj: |
Artery Research, Vol 12 (2015) |
| Druh dokumentu: |
article |
| ISSN: |
1876-4401 |
| DOI: |
10.1016/j.artres.2015.10.265 |
| Popis: |
Obesity is becoming a global epidemic and is associated with cardiovascular disease. Genetic factors play a significant role in the disease etiology but less is known about the interaction between genes and diet composition. This study is aimed to investigate the effect of diet and/or genotype on microcirculation in mice. Five groups of male mice (28 weeks) were examined using micro-ultrasound (Vevo2100, VisualSonics): 5 wild type mice on standard diet (WT_DS), 7 wild type mice on high-fat diet (WT_HF), 7 OB/+ mice on standard diet (OB/+_SD), 5 OB/+ mice on high-fat diet (OB/+_HF) and 4 OB/OB mice on standard diet (OB/OB_SD). The high-fat diet (45% energy as fat) groups were treated for 18 weeks before US scans. Infrarenal vasculature was imaged using Power-Doppler mode and Pulsed-Wave Doppler signals were acquired at the segmental level; Resistivity Index (RI) and Pulsatility Index (PI) were then assessed. Both RI and PI were significantly lower in WT_DS than in WT_HF (0.57±0.03vs0.67±0.06 and 0.86±0.04vs1.10±0.09, respectively). The same result was found for the comparison between OB/+_SD and OB/+_HF (0.63±0.06vs0.72±0.04 and 1±0.12vs1.22±0.09, respectively). RI and PI values were significantly different between WT_HF and OB/+_HF mice, while no differences were found for WT_DS-OB/+_DS, WT_HF-OB/OB_SD and OB/+_HF-OB/OB_SD comparisons. The high-fat diet has effects on the microvasculature of both WT and OB/+ mice. The two genotypes respond differently to the high-fat diet but not to the standard one. Moreover, if treated with high-fat diet, WT and OB/+ animals are not different from OB/OB mice (standard diet) in terms of microcirculation. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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