Induction of glioblastoma cell ferroptosis using combined treatment with chloramphenicol and 2-deoxy-d-glucose
Autor: | Kenji Miki, Mikako Yagi, Naoki Noguchi, Yura Do, Ryosuke Otsuji, Daisuke Kuga, Dongchon Kang, Koji Yoshimoto, Takeshi Uchiumi |
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Jazyk: | angličtina |
Rok vydání: | 2023 |
Předmět: | |
Zdroj: | Scientific Reports, Vol 13, Iss 1, Pp 1-12 (2023) |
Druh dokumentu: | article |
ISSN: | 2045-2322 50708414 |
DOI: | 10.1038/s41598-023-37483-5 |
Popis: | Abstract Glioblastoma, a malignant tumor, has no curative treatment. Recently, mitochondria have been considered a potential target for treating glioblastoma. Previously, we reported that agents initiating mitochondrial dysfunction were effective under glucose-starved conditions. Therefore, this study aimed to develop a mitochondria-targeted treatment to achieve normal glucose conditions. This study used U87MG (U87), U373, and patient-derived stem-like cells as well as chloramphenicol (CAP) and 2-deoxy-d-glucose (2-DG). We investigated whether CAP and 2-DG inhibited the growth of cells under normal and high glucose concentrations. In U87 cells, 2-DG and long-term CAP administration were more effective under normal glucose than high-glucose conditions. In addition, combined CAP and 2-DG treatment was significantly effective under normal glucose concentration in both normal oxygen and hypoxic conditions; this was validated in U373 and patient-derived stem-like cells. 2-DG and CAP acted by influencing iron dynamics; however, deferoxamine inhibited the efficacy of these agents. Thus, ferroptosis could be the underlying mechanism through which 2-DG and CAP act. In conclusion, combined treatment of CAP and 2-DG drastically inhibits cell growth of glioblastoma cell lines even under normal glucose conditions; therefore, this treatment could be effective for glioblastoma patients. |
Databáze: | Directory of Open Access Journals |
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