Relating the gut metagenome and metatranscriptome to immunotherapy responses in melanoma patients

Autor: Brandilyn A. Peters, Melissa Wilson, Una Moran, Anna Pavlick, Allison Izsak, Todd Wechter, Jeffrey S. Weber, Iman Osman, Jiyoung Ahn
Jazyk: angličtina
Rok vydání: 2019
Předmět:
Zdroj: Genome Medicine, Vol 11, Iss 1, Pp 1-14 (2019)
Druh dokumentu: article
ISSN: 1756-994X
DOI: 10.1186/s13073-019-0672-4
Popis: Abstract Background Recent evidence suggests that immunotherapy efficacy in melanoma is modulated by gut microbiota. Few studies have examined this phenomenon in humans, and none have incorporated metatranscriptomics, important for determining expression of metagenomic functions in the microbial community. Methods In melanoma patients undergoing immunotherapy, gut microbiome was characterized in pre-treatment stool using 16S rRNA gene and shotgun metagenome sequencing (n = 27). Transcriptional expression of metagenomic pathways was confirmed with metatranscriptome sequencing in a subset of 17. We examined associations of taxa and metagenomic pathways with progression-free survival (PFS) using 500 × 10-fold cross-validated elastic-net penalized Cox regression. Results Higher microbial community richness was associated with longer PFS in 16S and shotgun data (p
Databáze: Directory of Open Access Journals
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