DNA barcode reveals candidate species of Scinax and Ololygon (Anura: Hylidae) in Atlantic Forest

Autor: Lídia Nogueira, Luís Fernando da Silva Rodrigues Filho, Mirco Solé, Paulo Roberto Antunes de Mello Affonso, Sergio Siqueira, Iracilda Sampaio
Jazyk: angličtina
Rok vydání: 2022
Předmět:
Zdroj: Genetics and Molecular Biology, Vol 45, Iss 1 (2022)
Druh dokumentu: article
ISSN: 1678-4685
DOI: 10.1590/1678-4685-gmb-2021-0177
Popis: Abstract Molecular species delimitation methods are efficient tools to identify species, including the discovery of new taxa and cryptic organisms, thus being useful to biodiversity studies. In the present work, 16S mitochondrial sequences and cytochrome oxidase I (COI) were used to evaluate the richness of species in the genus Scinax and Ololygon from a biodiversity hotspot in Atlantic Forest. A total of 109 specimens formally belonging to eight species of Scinax and three species of Ololygon were collected in 13 localities along the state of Bahia (northeastern Brazil) and one site in Espírito Santo (southeastern Brazil). Of the Scinax species collected in this study, three were morphologically differentiated from other described species and identified as putative new species (Scinax sp.1, Scinax sp.2 and Scinax sp.3). The species delimitations were inferred using three different methods: ABGD, PTP and mPTP which allowed recognizing 11 Scinax species and five Ololygon species. Scinax sp. 1, Scinax sp. 2 and Scinax sp. 3, have been confirmed as new putative species and Ololygon argyreornata possibly contains cryptic species. We suggest additional studies, including morphological and bioacoustic data to validate these new putative species.
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