Spleen extracellular matrix provides a supportive microenvironment for β-cell function

Autor: Layasadat Khorsandi, Mahmoud Orazizadeh, Darioush Bijan Nejad, Abbas Heidari Moghadam, Freshteh Nejaddehbashi, Yousef Asadi Fard
Jazyk: angličtina
Rok vydání: 2022
Předmět:
Zdroj: Iranian Journal of Basic Medical Sciences, Vol 25, Iss 9, Pp 1159-1165 (2022)
Druh dokumentu: article
ISSN: 2008-3866
2008-3874
DOI: 10.22038/ijbms.2022.65233.14360
Popis: Objective(s): Type 1 diabetes mellitus is a common autoimmune and multifactorial disorder. Researchers have been interested in making a favorable islet-like tissue model for the treatment of diabetes. The main objective of this study was to determine the effects of the spleen extracellular matrix (S-ECM) on the function of the MIN6 cell line (a β-cell model). Materials and Methods: In this experimental research, Wistar rat spleens were decellularized by sodium dodecyl sulfate (SDS) and Triton X-100. S-ECM was characterized by histological assessments, scanning electron microscopy, determination of residua DNA, and examination of the mechanical tensile property. Then, MIN6 cells were seeded on S-ECM scaffold. Glucose-stimulated insulin secretion and mRNA expression of insulin-related genes were examined to confirm the function of the cells. Results: The main components of S-ECM such as collagen and glycosaminoglycan remained after decellularization. Furthermore, very low residual DNA and appropriate mechanical behavior of S-ECM provided an ideal extracellular microenvironment for the MIN6 cells. GSIS results showed that the seeded cells in S-ECM secreted more insulin than the traditional two-dimensional (2D) culture. The expression of specific insulin-related genes such as PDX-1, insulin, Maf-A, and Glut-2 in the recellularized scaffold was more significant than in the 2D traditional cultured cells. Also, MTT assay results showed that S-ECM were no cytotoxic effects on the MIN6 cells. Conclusion: These results collectively have evidenced that S-ECM is a suitable scaffold for stabilizing artificial pancreatic islands.
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