Chemical mutagenesis in head cabbage (Brassica oleracea var. capitata L.): IV DNA polymorphism

Autor: Tomlekova Nasya, Antonova Galina, Nikolova Vesselina, Petkova Valentina
Jazyk: English<br />Serbian
Rok vydání: 2006
Předmět:
Zdroj: Selekcija i Semenarstvo, Vol 12, Iss 1-2, Pp 95-100 (2006)
Druh dokumentu: article
ISSN: 0354-5881
2406-209X
Popis: ISSR method was applied for a detection of induced variability on a DNA level in M1 generation after Ethylmethan Sulphonate (EMS) chemical treatment in concentrations 0.5, 0.6 and 0.7% of seeds from head cabbage, Brassica oleraceae L, var. Capitata, cv. Ditmarsko. Different patterns of mutant plants obtained from the studied species were established by randomly selected primer sequences among tandem repeats. Polymorphisms were revealed amongM1 and control plants. Diverse DNA patterns of mutant plants were identified. As a result of the pre-breeding screening done in M1, altered plants were selected and seeds f or growing the next M2 generation were gathered.
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