| Autor: |
Markus Stoeckli, Han Wang, Dieter Staab, Frederic Grandjean, Fabian Sesterhenn, Christian Opitz |
| Jazyk: |
angličtina |
| Rok vydání: |
2024 |
| Předmět: |
|
| Zdroj: |
SLAS Technology, Vol 29, Iss 6, Pp 100216- (2024) |
| Druh dokumentu: |
article |
| ISSN: |
2472-6303 |
| DOI: |
10.1016/j.slast.2024.100216 |
| Popis: |
Tracking chemical reactions by measuring incurred mass shifts upon successful binding is a direct and attractive alternative to existing assays based on chemical tags. Traditional methods use liquid chromatography-mass spectrometry (LC-MS), and because the required buffers are not amenable to direct MS injection, sample pre-treatment is needed to desalt. This leads to analysis times from ten seconds to minutes per sample, limiting throughput and preventing widespread application.Combining an acoustic ejection (AE) interface with a time-of-flight mass spectrometer (MS) removes this bottleneck, as samples can be directly introduced at rates of up to one second per sample. This article describes a complete workflow for measuring the covalent binding of compounds to proteins in real-time, from assay to data evaluation. It is noteworthy that this is the first instance of using SCIEX Echo® MS+ system with ZenoTOF 7600 system to study the kinetic regimes of covalent binding. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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