Haplotype-resolved genome assembly of Coriaria nepalensis a non-legume nitrogen-fixing shrub

Autor: Shi-Wei Zhao, Jing-Fang Guo, Lei Kong, Shuai Nie, Xue-Mei Yan, Tian-Le Shi, Xue-Chan Tian, Hai-Yao Ma, Yu-Tao Bao, Zhi-Chao Li, Zhao-Yang Chen, Ren-Gang Zhang, Yong-Peng Ma, Yousry A. El-Kassaby, Ilga Porth, Wei Zhao, Jian-Feng Mao
Jazyk: angličtina
Rok vydání: 2023
Předmět:
Zdroj: Scientific Data, Vol 10, Iss 1, Pp 1-11 (2023)
Druh dokumentu: article
ISSN: 2052-4463
DOI: 10.1038/s41597-023-02171-6
Popis: Abstract Coriaria nepalensis Wall. (Coriariaceae) is a nitrogen-fixing shrub which forms root nodules with the actinomycete Frankia. Oils and extracts of C. nepalensis have been reported to be bacteriostatic and insecticidal, and C. nepalensis bark provides a valuable tannin resource. Here, by combining PacBio HiFi sequencing and Hi-C scaffolding techniques, we generated a haplotype-resolved chromosome-scale genome assembly for C. nepalensis. This genome assembly is approximately 620 Mb in size with a contig N50 of 11 Mb, with 99.9% of the total assembled sequences anchored to 40 pseudochromosomes. We predicted 60,862 protein-coding genes of which 99.5% were annotated from databases. We further identified 939 tRNAs, 7,297 rRNAs, and 982 ncRNAs. The chromosome-scale genome of C. nepalensis is expected to be a significant resource for understanding the genetic basis of root nodulation with Frankia, toxicity, and tannin biosynthesis.
Databáze: Directory of Open Access Journals