| Autor: |
S Ujwal, S Sabeena, R Bhaskar, G D'Souza, D Santhosha, A Auti, R Kumar, S Ramachandran, Maity Hindol, A Aithal, Y Bhandari, A Jazeel, G Arunkumar |
| Jazyk: |
angličtina |
| Rok vydání: |
2019 |
| Předmět: |
|
| Zdroj: |
Journal of Vector Borne Diseases, Vol 56, Iss 3, Pp 231-236 (2019) |
| Druh dokumentu: |
article |
| ISSN: |
0972-9062 |
| DOI: |
10.4103/0972-9062.289402 |
| Popis: |
Background & objectives: Dengue is a major public health problem in northeast India where the majority of the cases go unreported and undiagnosed. Even though all four dengue serotypes are reported, there is a dearth of information on genetic diversity. The present cross-sectional study was undertaken during 2016–17 to determine the genetic variance of dengue virus serotype 2 (DENV-2) based on the envelope (E) glycoprotein gene. Methods: The serum samples collected from the northeast parts of India, as a part of hospital-based acute febrile illness surveillance, were serotyped. Viral RNA was extracted from DENV-2 serum samples using QIAquick® RNA Extraction Kit. The E gene was amplified by conventional reverse-transcriptase polymerase chain reaction (RT-PCR) and the PCR products were sequenced. Results: The E glycoprotein gene of nine serum samples with high viral RNA concentration (Ct |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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