Miniaturizable Chemiluminescence System for ATP Detection in Water

Autor:	Giuseppe E. Capuano, Domenico Corso, Roberta Farina, Gianni Pezzotti Escobar, Giuseppe A. Screpis, Maria Anna Coniglio, Sebania Libertino
Jazyk:	angličtina
Rok vydání:	2024
Předmět:	ATP bioluminescence detection silicon photomultiplier bacterial charge detection miniaturized sensing system diffusion time Chemical technology TP1-1185
Zdroj:	Sensors, Vol 24, Iss 12, p 3921 (2024)
Druh dokumentu:	article
ISSN:	1424-8220
DOI:	10.3390/s24123921
Popis:	We present the design, fabrication, and testing of a low-cost, miniaturized detection system that utilizes chemiluminescence to measure the presence of adenosine triphosphate (ATP), the energy unit in biological systems, in water samples. The ATP–luciferin chemiluminescent solution was faced to a silicon photomultiplier (SiPM) for highly sensitive real-time detection. This system can detect ATP concentrations as low as 0.2 nM, with a sensitivity of 79.5 A/M. Additionally, it offers rapid response times and can measure the characteristic time required for reactant diffusion and mixing within the reaction volume, determined to be 0.3 ± 0.1 s. This corresponds to a diffusion velocity of approximately 44 ± 14 mm2/s.
Databáze:	Directory of Open Access Journals
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