Evaluation of method for phthalate extraction from milk related to milk dilution ratio
| Autor: | Milojković Danica S., Anđelković Darko H., Kocić Gordana M., Anđelković Tatjana D. |
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| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: | |
| Zdroj: | Journal of the Serbian Chemical Society, Vol 80, Iss 8, Pp 983-996 (2015) |
| Druh dokumentu: | article |
| ISSN: | 0352-5139 1820-7421 14120402 |
| DOI: | 10.2298/JSC141204028M |
| Popis: | Liquid-liquid extraction techniques were compared coupled with gas chromatography-mass spectrometry (GC-MS), for the extraction and the determination of four phthalates: dimethyl phthalate (DMP), di-n-butyl phthalate (DBP), benzyl butyl phthalate (BBP) and di-(2-ethylhexyl) phthalate (DEHP) in six different kinds of milk-based samples. Extraction factors: sample preparation, organic solvent type and volume, salt effect, agitation and the extraction time were optimized. The ion of base peaks (m/z 149 for DBP, BBP and DEHP and m/z 163 for DMP) for investigated phthalates were selected for the screening studies. The acquisition was performed at the selected ion monitoring mode. The MSD response for GC-MS phthalate calibration standards was linear between 0.25 and 2.50 μg mL-1 with calculated LODs between 0.01 μg mL-1 to 0.04 μg mL-1 and LOQs of 0.05 μg mL-1 to 0.12 μg mL-1, while repeatability was between 1.7 % to 4.9 % RSD. The study demonstrated an increase of the recovery of less polar phthalates in matrix milk standards by matrix dilution. Recovery for hydrophilic phthalates, like DMP, was not changed by matrix dilution and it was continuously low for the investigated method. Two spiking levels were tested for influence of matrix dilution on phthalate recovery, showed the same trend. [Projekat Ministarstva nauke Republike Srbije, br. 31060] |
| Databáze: | Directory of Open Access Journals |
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