The Effect of Wheat Sprout Extract on Skin Injury Following Injection of Lead Acetate in Rat
| Autor: | HR Moradi, H Morovvati, M Adibmoradi, H Najafzadeh Varzi |
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| Jazyk: | perština |
| Rok vydání: | 2017 |
| Předmět: | |
| Zdroj: | Armaghane Danesh Bimonthly Journal, Vol 22, Iss 2, Pp 161-175 (2017) |
| Druh dokumentu: | article |
| ISSN: | 1728-6514 1728-6506 |
| Popis: | Abstract Background & aim: Skin is constantly exposed to environmental contaminants such as heavy metals (lead).Medicinal plants have been concern for the treatment of of human pain. and pain. Wheat Sprout is one of medicinal plants which are rich in vitamins, minerals and powerful antioxidant compounds respectively. The aim of this study was to evaluate the effect of wheat Sprout extract on tissue texture following injection of lead acetate in rats. Methods: Thirty healthy adult Wistar rats were divided randomly into six groups: Control group received 1 ml/kg/day of normal saline, group 2 received 20 mg/kg/day of lead acetate intraperitoneally respectively, group 3 and group 4 received 100 mg/kg/day and 200 mg/kg/day of wheat sprout extract by gavage feeding, group 5 and group 6 received 100 mg/kg/day and 200 mg/kg/day of wheat sprout extract by gavage feeding along with 20 mg/kg/day of lead acetate intraperitoneally. After five weeks, skin tissue of dorsal region and blood samples were collected for histomorphometric studies and serum assessment. Serum samples were tested for determining antioxidant activity (AOA) based on power ferric reduction antioxidant (FRAP) assay and peroxidation of lipids by measuring malondialdehyde (MDA). The 5 to 6 μm thickness sections were made using paraffin embedding method after stained by hematoxylin and eosin, safranin and masson trichrome. For microscopic study, Dino-Lite digital lens and Dino Capture 2 Software were used. Results: The lead significantly decreased the total thickness of the skin, the dermal layer, hypoderm, the number and maximum depth of hair follicles and the epidermis pod thickness of the hair root compared with the control group (p |
| Databáze: | Directory of Open Access Journals |
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