Isolation and Functional Analysis of ZmLTP3, a Homologue to Arabidopsis LTP3

Autor: Guo-Hui Ma, Xiao-Hai Tian, Hua-Wen Zou, Zhi-Xin Li
Jazyk: angličtina
Rok vydání: 2013
Předmět:
Zdroj: International Journal of Molecular Sciences, Vol 14, Iss 3, Pp 5025-5035 (2013)
Druh dokumentu: article
ISSN: 1422-0067
DOI: 10.3390/ijms14035025
Popis: Plant lipid transfer proteins (LTPs) are encoded by multigene families and play important roles in plant physiology. One full-length cDNA encoding an Arabidopsis LTP3 homologue was isolated from maize by RT-PCR and named as ZmLTP3. RT-PCR analysis indicated that the ZmLTP3 expression is induced by salicylic acid (SA), mannitol and salt. Furthermore, in different tissues the ZmLTP3 displayed different expression patterns, indicating that ZmLTP3 may play multiple roles in stress resistance. Over-expression of ZmLTP3 in wild-type Arabidopsis resulted in the increased salt tolerance. Under salt stress condition, compared to wild-type (WT) plants, transgenic Arabidopsis grew better, had higher seedling fresh (FW), dry weight (DW), seed yields, proline content and lower MDA content and relative electric conductivity level. Our results suggest that maize ZmLTP3 might encode a member of LTPs family and play roles in salt resistance.
Databáze: Directory of Open Access Journals