Induction of Adventitious buds from stem explants in Aoectochilus formosanus

Autor: Xue Yingbin, Xiao Fengyi, Li Xiaohao, Chen Huamei, Rao Gangshun, Liu Ying
Jazyk: English<br />French
Rok vydání: 2019
Předmět:
Zdroj: E3S Web of Conferences, Vol 131, p 01131 (2019)
Druh dokumentu: article
ISSN: 2267-1242
DOI: 10.1051/e3sconf/201913101131
Popis: In order to explore the optimal culture conditions for adventitious bud regeneration of stem explants in Aoectochilus formosanus, the stem segments from the sterile seedlings were used as explants, and different concentrations of diethyl aminoethyl hexanoate (DA-6), kinetin (KT), Cu2+ and glutamine (Gln) were separately added into MS medium containing 6-benzylaminopurine (6-BA) and naphthaleneacetic acid (NAA), and the induction rate and the induction multiple of adventitious buds were recorded and analyzed. The results showed that the regeneration of adventitious buds could be promoted, when 2 mg/L DA-6, 0.4 mg/L KT and 15 mg/L Gln were added in mediums. However, the effect of Cu2+ on the regeneration of adventitious buds in A. formosanus was enhanced by low concentration and suppressed by high concentration, and the best concentration of Cu2+ was 5 mg/L.
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