| Autor: |
Haiyan Wu, Zuochao Yao, Hongkun Li, Laihai Zhang, Yuying Zhao, Yongwei Li, Yating Wu, Zhenchun Zhang, Jiali Xie, Feixue Ding, Hongming Zhu |
| Jazyk: |
angličtina |
| Rok vydání: |
2024 |
| Předmět: |
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| Zdroj: |
Journal of Nanobiotechnology, Vol 22, Iss 1, Pp 1-21 (2024) |
| Druh dokumentu: |
article |
| ISSN: |
1477-3155 |
| DOI: |
10.1186/s12951-024-02541-1 |
| Popis: |
Abstract Skin aging is characterized by the disruption of skin homeostasis and impaired skin injury repair. Treatment of aging skin has long been limited by the unclear intervention targets and delivery techniques. Engineering extracellular vesicles (EVs) as an upgraded version of natural EVs holds great potential in regenerative medicine. In this study, we found that the expression of the critical antioxidant and detoxification gene Gstm2 was significantly reduced in aging skin. Thus, we constructed the skin primary fibroblasts-derived EVs encapsulating Gstm2 mRNA (EVs Gstm2 ), and found that EVs Gstm2 could significantly improve skin homeostasis and accelerate wound healing in aged mice. Mechanistically, we found that EVs Gstm2 alleviated oxidative stress damage of aging dermal fibroblasts by modulating mitochondrial oxidative phosphorylation, and promoted dermal fibroblasts to regulate skin epidermal cell function by paracrine secretion of Nascent Polypeptide-Associated Complex Alpha subunit (NACA). Furthermore, we confirmed that NACA is a novel skin epidermal cell protective molecule that regulates skin epidermal cell turnover through the ROS-ERK-ETS-Cyclin D pathway. Our findings demonstrate the feasibility and efficacy of EVs-mediated delivery of Gstm2 for aged skin treatment and unveil novel roles of GSTM2 and NACA for improving aging skin. Graphical Abstract |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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