| Autor: |
Morgane Mailhe, Davide Ricaboni, Véronique Vitton, Jean-Michel Gonzalez, Dipankar Bachar, Grégory Dubourg, Frédéric Cadoret, Catherine Robert, Jérémy Delerce, Anthony Levasseur, Pierre-Edouard Fournier, Emmanouil Angelakis, Jean-Christophe Lagier, Didier Raoult |
| Jazyk: |
angličtina |
| Rok vydání: |
2018 |
| Předmět: |
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| Zdroj: |
BMC Microbiology, Vol 18, Iss 1, Pp 1-11 (2018) |
| Druh dokumentu: |
article |
| ISSN: |
1471-2180 |
| DOI: |
10.1186/s12866-018-1304-7 |
| Popis: |
Abstract Background Most studies on the human microbiota have analyzed stool samples, although a large proportion of the absorption of nutrients takes place in upper gut tract. We collected samples from different locations along the entire gastrointestinal tract from six patients who had simultaneously undergone upper endoscopy and colonoscopy, to perform a comprehensive analysis using culturomics with matrix assisted laser desorption ionisation - time of flight (MALDI-TOF) identification and by metagenomics targeting the 16S ribosomal ribonucleic acid (rRNA) gene. Results Using culturomics, we isolated 368 different bacterial species, including 37 new species. Fewer species were isolated in the upper gut: 110 in the stomach and 106 in the duodenum, while 235 were isolated from the left colon (p |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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