Whole-Genome Sequencing Reveals Recent Transmission of Multidrug-Resistant Mycobacterium tuberculosis CAS1-Kili Strains in Lusaka, Zambia

Autor: Joseph Yamweka Chizimu, Eddie Samuneti Solo, Precious Bwalya, Wimonrat Tanomsridachchai, Herman Chambaro, Misheck Shawa, Thoko Flav Kapalamula, Patrick Lungu, Yukari Fukushima, Victor Mukonka, Jeewan Thapa, Chie Nakajima, Yasuhiko Suzuki
Jazyk: angličtina
Rok vydání: 2021
Předmět:
Zdroj: Antibiotics, Vol 11, Iss 1, p 29 (2021)
Druh dokumentu: article
ISSN: 11010029
2079-6382
DOI: 10.3390/antibiotics11010029
Popis: Globally, tuberculosis (TB) is a major cause of death due to antimicrobial resistance. Mycobacterium tuberculosis CAS1-Kili strains that belong to lineage 3 (Central Asian Strain, CAS) were previously implicated in the spread of multidrug-resistant (MDR)-TB in Lusaka, Zambia. Thus, we investigated recent transmission of those strains by whole-genome sequencing (WGS) with Illumina MiSeq platform. Twelve MDR CAS1-Kili isolates clustered by traditional methods (MIRU-VNTR and spoligotyping) were used. A total of 92% (11/12) of isolates belonged to a cluster (≤12 SNPs) while 50% (6/12) were involved in recent transmission events, as they differed by ≤5 SNPs. All the isolates had KatG Ser315Thr (isoniazid resistance), EmbB Met306 substitutions (ethambutol resistance) and several kinds of rpoB mutations (rifampicin resistance). WGS also revealed compensatory mutations including a novel deletion in embA regulatory region (−35A > del). Several strains shared the same combinations of drug-resistance-associated mutations indicating transmission of MDR strains. Zambian strains belonged to the same clade as Tanzanian, Malawian and European strains, although most of those were pan-drug-susceptible. Hence, complimentary use of WGS to traditional epidemiological methods provides an in-depth insight on transmission and drug resistance patterns which can guide targeted control measures to stop the spread of MDR-TB.
Databáze: Directory of Open Access Journals
Externí odkaz: