In Vitro Maturation of Viable Islets from Partially Digested Young Pig Pancreas
| Autor: | Morgan Lamb, Kelly Laugenour, Ouwen Liang, Michael Alexander, Clarence E. Foster, Jonathan R. T. Lakey Ph.D. |
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| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: | |
| Zdroj: | Cell Transplantation, Vol 23 (2014) |
| Druh dokumentu: | article |
| ISSN: | 0963-6897 1555-3892 |
| DOI: | 10.3727/096368912X662372 |
| Popis: | Isolation of islets from market-sized pigs is costly, with considerable islet losses from fragmentation occurring during isolation and tissue culture. Fetal and neonatal pigs yield insulin unresponsive islet-like cell clusters that become glucose-responsive after extended periods of time. Both issues impact clinical applicability and commercial scale-up. We have focused our efforts on a cost-effective scalable method of isolating viable insulin-responsive islets. Young Yorkshire pigs (mean age 20 days, range 4–30 days) underwent rapid pancreatectomy ( |
| Databáze: | Directory of Open Access Journals |
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