| Autor: |
Andrew W. Jones, Helen R. Flynn, Frank Uhlmann, Ambrosius P. Snijders, Sandra A. Touati |
| Jazyk: |
angličtina |
| Rok vydání: |
2020 |
| Předmět: |
|
| Zdroj: |
STAR Protocols, Vol 1, Iss 1, Pp 100022- (2020) |
| Druh dokumentu: |
article |
| ISSN: |
2666-1667 |
| DOI: |
10.1016/j.xpro.2020.100022 |
| Popis: |
Summary: Amine-reactive Tandem Mass Tag 10plex (TMT10plex) labeling permits multiplexed protein identification and quantitative analysis by tandem mass spectrometry (MS/MS). We have used this technology to label 20 Saccharomyces cerevisiae samples collected in a time-resolved manner from a wild-type and phosphatase mutant background to characterize phosphoproteome dynamics. Here, we provide a detailed protocol for biological and mass spectrometry sample preparation and analysis. For complete details on the use and execution of this protocol, please refer to Touati et al. (2019). |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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