| Autor: |
Scott M. Krummey, Anna B. Morris, Jesica R. Jacobs, Donald J. McGuire, Satomi Ando, Katherine P. Tong, Weiwen Zhang, Jennifer Robertson, Sara A. Guasch, Koichi Araki, Christian P. Larsen, Brian D. Evavold, Haydn T. Kissick, Mandy L. Ford |
| Jazyk: |
angličtina |
| Rok vydání: |
2020 |
| Předmět: |
|
| Zdroj: |
Cell Reports, Vol 30, Iss 5, Pp 1282-1291.e5 (2020) |
| Druh dokumentu: |
article |
| ISSN: |
2211-1247 |
| DOI: |
10.1016/j.celrep.2020.01.016 |
| Popis: |
Summary: The identity of CD45 isoforms on the T cell surface changes following the activation of naive T cells and impacts intracellular signaling. In this study, we find that the anti-viral memory CD8+ T pool is unexpectedly comprised of both CD45RBhi and CD45RBlo populations. Relative to CD45RBlo memory T cells, CD45RBhi memory T cells have lower affinity and display greater clonal diversity, as well as a persistent CD27hi phenotype. The CD45RBhi memory population displays a homeostatic survival advantage in vivo relative to CD45RBlo memory, and long-lived high-affinity cells that persisted long term convert from CD45RBlo to CD45RBhi. Human CD45RO+ memory is comprised of both CD45RBhi and CD45RBlo populations with distinct phenotypes, and antigen-specific memory to two viruses is predominantly CD45RBhi. These data demonstrate that CD45RB status is distinct from the conventional central/effector T cell memory classification and has potential utility for monitoring and characterizing pathogen-specific CD8+ T cell responses. : Krummey et al. show that viral CD8+ T cell memory has heterogeneous CD45 isoform expression. Low-affinity CD8+ T cells have high CD45RB expression and a CD27hiCD62Lhi phenotype relative to high-affinity CD45RBlo CD8+ T cells, which possess an effector-like phenotype. CD45RBhi cells survive better under homeostatic conditions in vivo. Keywords: T cell memory, TCR affinity, CD45 |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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