| Autor: |
Rachel Krabacher, Steve Kim, Yen Ngo, Joseph Slocik, Christina Harsch, Rajesh Naik |
| Jazyk: |
angličtina |
| Rok vydání: |
2021 |
| Předmět: |
|
| Zdroj: |
Chemosensors, Vol 9, Iss 9, p 245 (2021) |
| Druh dokumentu: |
article |
| ISSN: |
2227-9040 |
| DOI: |
10.3390/chemosensors9090245 |
| Popis: |
Peptides can recognize and selectively bind to a wide variety of materials dependent on both their surface properties and the environment. Biopanning with phage or cell peptide display libraries can identify material-specific binding peptides. However, the limitations with sequence diversity of traditional bacteriophage (phage) display libraries and loss of unique phage clones during the amplification cycles results in a smaller pool of peptide sequences identified. False positive sequences tend to emerge during the biopanning process due to highly proliferating, yet nonspecific, phages. In order to overcome this limitation of traditional biopanning methodology, a modified method using high-throughput next generation sequencing (HTS) was tested to select for unique peptides specific to two types of single wall carbon nanotube (SWNTs) sources with varying diameter distribution and chirality. Here, the process, analysis, and characterization of peptide sequences identified using the modified method is further described and compared to a peptide identified in literature using the traditional method. Selected sequences from this study were incorporated in a SWNT dispersion experiment to probe their selectivity to the nanotube diameter. We show that NHTS can uncover unique binding sequences that might have otherwise been lost during the traditional biopanning method. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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