| Autor: |
Wang Xiaolan, Yang Hanyi, Zhang Yimeng, Liu Sida, Chen Chengming, Xie Tingke, Chen Yixuan, Ning Jiayi, Han Jing |
| Jazyk: |
angličtina |
| Rok vydání: |
2025 |
| Předmět: |
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| Zdroj: |
Guoji Yanke Zazhi, Vol 25, Iss 3, Pp 359-364 (2025) |
| Druh dokumentu: |
article |
| ISSN: |
1672-5123 |
| DOI: |
10.3980/j.issn.1672-5123.2025.3.04 |
| Popis: |
AIM: To investigate the potential inhibitory effect of pterostilbene on the endothelial-to-mesenchymal transition(EndMT)induced by high glucose conditions in human retinal microvascular endothelial cells(HRMECs).METHODS: The optimal concentration of pterostilbene for treating HRMECs was determined using the CCK-8 assay, with 12.5 and 25 μmol/L concentrations selected for subsequent experiments. Four experimental groups were established: control group, high glucose group, high glucose combined with 12.5 μmol/L pterostilbene treatment group, and high glucose combined with 25 μmol/L pterostilbene treatment group. The expression levels of HDAC7 and EndMT-associated markers were detected via Western blot analysis. Cell migration ability was assessed using Transwell migration assays and scratch wound healing tests, while vasculogenic capability was evaluated through tube formation assays.RESULTS: The CCK-8 assay revealed that pterostilbene at a concentration of 22.07 μmol/L inhibited 50% of cell viability in HRMECs. Western blot analysis demonstrated that compared with the control group, the expression levels of HDAC7, ZEB1, Vimentin, and Snail were significantly upregulated in HRMECs cultured in high glucose(all P |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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