| Autor: |
Bogna J. Smug, Monika Opalek, Maks Necki, Dominika Wloch‐Salamon |
| Jazyk: |
angličtina |
| Rok vydání: |
2024 |
| Předmět: |
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| Zdroj: |
Methods in Ecology and Evolution, Vol 15, Iss 2, Pp 301-307 (2024) |
| Druh dokumentu: |
article |
| ISSN: |
2041-210X |
| DOI: |
10.1111/2041-210X.14269 |
| Popis: |
Abstract The duration of lag phase can be used as an organismal fitness marker; however, it is often underreported as its estimation may be challenging and method and parameters dependent. Moreover, there are no publicly available tools to calculate lag duration by different methods. We developed a shiny‐based web application (https://microbialgrowth.shinyapps.io/lag_calulator/) where the lag duration can be calculated based on the user‐specified growth curve data, and for various explicitly specified methods, parameters and data preprocessing techniques. Additionally, we release an R package ‘miLAG’ that can be further customised and developed. We also describe in short the assumptions, advantages and disadvantages of the most popular lag calculation methods and propose a decision tree to choose a method most suited to one's data. Finally, we show some working examples of how to calculate lag duration using our shiny server. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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