Quantitative Analysis of Streptococcus mutans, Streptococcus sobrinus and Streptococcus sanguinis and their Association with Early Childhood Caries
| Autor: | Kemthong Mitrakul, Boontarika Akarapipatkul, Piamporn Thammachat |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: | |
| Zdroj: | Journal of Clinical and Diagnostic Research, Vol 14, Iss 2, Pp ZC23-ZC28 (2020) |
| Druh dokumentu: | article |
| ISSN: | 2249-782X 0973-709X |
| DOI: | 10.7860/JCDR/2020/43086.13513 |
| Popis: | Introduction: Severe Early Childhood Caries (S-ECC) remains highly prevalent worldwide. Streptococcus mutans and Streptococcus sobrinus are causative pathogens of dental caries and strongly involved in plaque or oral biofilm formation. Streptococcus sanguinis has antagonistic relationship with S. mutans and it might delay the colonisation of S. mutans in the oral cavities. Aim: To quantify S. mutans, S. sobrinus and S. sanguinis between 2 groups {S-ECC and Caries Free (CF)} of Thai children and to analyse the association between these bacteria and caries-related factors. Materials and Methods: Supra-gingival overnight plaque samples were collected from 120 Thai children aged 2-5 years (S-ECC=CF=60) using sterile toothpicks and released in 1 mL of TrisBase and EDTA buffer from January 2015 to December 2017. They were asked to expectorate saliva into a cup, plaque and gingival indices and dmft scores were recorded, parent’s demographic and children’s oral hygiene care and diet were assessed using questionnaire. DNA extraction and quantitative real-time PCR was performed. Different amounts of each bacterium were analysed by Mann-Whitney U test (p |
| Databáze: | Directory of Open Access Journals |
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