Low acyl gellan gum immobilized Lactobacillus bulgaricus T15 produce d-lactic acid from non-detoxified corn stover hydrolysate
Autor: | Yongxin Guo, Yuru Zhao, Yuan Gao, Gang Wang, Yixin Zhao, Jiejing Zhang, Yanli Li, Xiqing Wang, Juan Liu, Guang Chen |
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Jazyk: | angličtina |
Rok vydání: | 2023 |
Předmět: | |
Zdroj: | Biotechnology for Biofuels and Bioproducts, Vol 16, Iss 1, Pp 1-10 (2023) |
Druh dokumentu: | article |
ISSN: | 2731-3654 |
DOI: | 10.1186/s13068-023-02292-5 |
Popis: | Abstract Straw biorefinery offers economical and sustainable production of chemicals. The merits of cell immobilization technology have become the key technology to meet d-lactic acid production from non- detoxified corn stover. In this paper, Low acyl gellan gum (LA-GAGR) was employed first time for Lactobacillus bulgaricus T15 immobilization and applied in d-lactic acid (D-LA) production from non-detoxified corn stover hydrolysate. Compared with the conventional calcium alginate (E404), LA-GAGR has a hencky stress of 82.09 kPa and excellent tolerance to 5-hydroxymethylfurfural (5-HMF), ferulic acid (FA), and vanillin. These features make LA-GAGR immobilized T15 work for 50 days via cell-recycle fermentation with D-LA yield of 2.77 ± 0.27 g/L h, while E404 immobilized T15 can only work for 30 days. The production of D-LA from non-detoxified corn stover hydrolysate with LA-GAGR immobilized T15 was also higher than that of free T15 fermentation and E404 immobilized T15 fermentation. In conclusion, LA-GAGR is an excellent cell immobilization material with great potential for industrial application in straw biorefinery industry. Graphical Abstract |
Databáze: | Directory of Open Access Journals |
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