| Autor: |
Biyun Zheng, Kyung-Pil Ko, Xuefen Fang, Xiaozhong Wang, Jie Zhang, Sohee Jun, Bong-Jun Kim, Wenyi Luo, Moon Jong Kim, Youn-Sang Jung, Christopher L. Cervantes, Jae-Il Park |
| Jazyk: |
angličtina |
| Rok vydání: |
2021 |
| Předmět: |
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| Zdroj: |
iScience, Vol 24, Iss 12, Pp 103440- (2021) |
| Druh dokumentu: |
article |
| ISSN: |
2589-0042 |
| DOI: |
10.1016/j.isci.2021.103440 |
| Popis: |
Summary: Organoids mimic the physiologic and pathologic events of organs. However, no consensus on esophageal organoid (EO) culture methods has been reached. Moreover, organoid models reproducing esophageal squamous cell carcinoma (ESCC) initiation have been unavailable. Herein, we sought to develop an esophageal minimum essential organoid culture medium (E-MEOM) for culturing murine EOs and establishing an early ESCC model. We formulated E-MEOM to grow EOs from a single cell with clonal expansion, maintenance, and passage. We found that EOs cultured in E-MEOM were equivalent to the esophageal epithelium by histological analysis and transcriptomic study. Trp53 knockout and KrasG12D expression in EOs induced the development of esophageal squamous neoplasia, an early lesion of ESCC. Here we propose the new formula for EO culture with minimum components and the organoid model recapitulating ESCC initiation, laying the foundation for ESCC research and drug discovery. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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