Transcriptional analysis of mouse wounds grafted with human mesenchymal stem cells and platelets

Autor: Jennyfer Levoux, Peggy Lafuste, Anne-Marie Rodriguez
Jazyk: angličtina
Rok vydání: 2021
Předmět:
Zdroj: STAR Protocols, Vol 2, Iss 3, Pp 100650- (2021)
Druh dokumentu: article
ISSN: 2666-1667
DOI: 10.1016/j.xpro.2021.100650
Popis: Summary: Platelet preparations are commonly used in the clinic in combination with mesenchymal stem cells (MSCs) to improve their wound healing capacity and optimize their therapeutic efficacy following their delivery into diseased tissues. To investigate the mechanisms by which platelets enhance the repair properties of MSCs, we detail a protocol using a humanized mouse model for excisional wounds to study by reverse transcription real-time PCR whether human platelets alter the therapeutic efficacy of grafted human MSCs.For complete details on the use and execution of this protocol, please refer to Levoux et al. (2021).
Databáze: Directory of Open Access Journals