The effect of cryoprotectant on the viability and motility of goldfish Carassius aurtus sperm after 48 hours refrigerated
| Autor: | Nurlaili Nurlaili, Itsnatani Salma, Kartini Eriani, Siti Maulida, Zainal Abidin Muchlisin |
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| Jazyk: | angličtina |
| Rok vydání: | 2022 |
| Předmět: | |
| Zdroj: | Depik Jurnal, Vol 11, Iss 3, Pp 327-332 (2022) |
| Druh dokumentu: | article |
| ISSN: | 2089-7790 2502-6194 |
| DOI: | 10.13170/depik.11.3.28480 |
| Popis: | The principle of sperm preservation is to prolong the viability of spermatozoa by reducing the degree of metabolism during storage at low temperatures, and one method involved was a cryoprotectant as a sperm protector. This study aimed to determine the best type of cryoprotectant for refrigeration storage of goldfish Carrasius auratus sperm. A completely randomized design was used, and five types of cryoprotectant were tested, namely Methanol, Ethanol, Ethylene Glycol, Glycerol, and DMSO at a 10% concentration combined with 15% egg yolks. The sperm was diluted with Ringer’s solution and stored at 4⁰C for 48 hours. The ANOVA test revealed that cryoprotectant significantly affected the motility and viability of sperm after 48 hours of refrigeration (P0.05). The results showed that 10% DMSO combined with 15% egg yolk could maintain sperm motility and viability better than other treatments, with values of 85.80% and 86.50%, respectively, which were significantly different from the other treatments. Glycerol produced the second-best sperm quality with motility and viability values of 71.13% and 74.13%, respectively. Therefore, DMSO is the most effective cryoprotectant for refrigeration storage of goldfish sperm. |
| Databáze: | Directory of Open Access Journals |
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