Effects of Hibiscus sabdariffa L. extract on multispecies Porphyromonas gingivalis and Treponema denticola biofilms in vitro
| Autor: | Angela Winson, Trijani Suwandi, Olivia Nauli Komala |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2023 |
| Předmět: | |
| Zdroj: | Scientific Dental Journal, Vol 7, Iss 1, Pp 26-32 (2023) |
| Druh dokumentu: | article |
| ISSN: | 2580-6548 2541-321X |
| DOI: | 10.4103/SDJ.SDJ_4_23 |
| Popis: | Background: Porphyromonas gingivalis and Treponema denticola are bacteria that play a crucial role in periodontitis. Hibiscus sabdariffa L. contains flavonoids, alkaloids, saponins, and tannins; compounds which are expected to inhibit biofilm formation with minimal side effects. Objectives: To determine the secondary metabolite compounds present in H. sabdariffa L. and its effect on multispecies biofilms formation consisted of P. gingivalis and T. denticola. Methods: This study used H. sabdariffa L. calyx extract with concentrations of 100%, 50%, 25%, 12.5%, 6.25%, 3.125%, and 1.5625%. The positive control used in this study was 0.2% chlorhexidine, and the negative control was brain heart infusion broth. The bacterial biofilm was grown for 1, 6, and 24 hours. Results: Hibiscus sabdariffa L. 1.5625% extracted in a 1-h incubation period, 6.25% extracted in a 6-h incubation period, and 3.25% extracted in a 24-h incubation period are more effective than chlorhexidine 0.2% in inhibiting multispecies P. gingivalis and T. denticola biofilms (P < 0.05). Conclusion: Hibiscus sabdariffa L. calyx extract significantly inhibits multispecies P. gingivalis and T. denticola biofilm with the most effective concentration at 100% at 1-h, 6-h, and 24-h incubation periods. Future studies are needed to test the toxicity of H. sabdariffa L. on cells in the oral cavity. |
| Databáze: | Directory of Open Access Journals |
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