Enhanced in vitro cytotoxicity and intracellular uptake of Genipin via loaded on Nano-Liposomes made from soy lecithin in MCF-7 cells
| Autor: | Tahereh Naseriyeh, Tayebeh Noori, Hosna Alvandi, Hossein Zhaleh, Leila Behbood, Alireza Shamsi, Faranak Aghaz, Elham Arkan |
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| Jazyk: | angličtina |
| Rok vydání: | 2022 |
| Předmět: | |
| Zdroj: | Nanomedicine Journal, Vol 9, Iss 1, Pp 67-76 (2022) |
| Druh dokumentu: | article |
| ISSN: | 2322-3049 2322-5904 |
| DOI: | 10.22038/nmj.2021.60524.1626 |
| Popis: | Objective(s): As an alternative to chemical drugs, natural compounds such as Genipin can reduce toxicity and side effects. In recent years, Genipin's antioxidant properties have been considered a potential cancer treatment. Therefore, the present study investigated anti-cancer activity of newly formulated nano-liposomal loaded Genipin, made from soy lecithin, against MCF-7 cancer cell line. Materials and Methods: After synthesis, the physicochemical properties of the liposomes were confirmed by Dynamic light scattering (DLS), Scanning Electron Microscopy (SEM), Fourier-transform infrared spectroscopy (FTIR), and UV-vis spectrophotometry. Results: Our results showed that the prepared nano-liposome had a diameter of 166.2 nm. Its Zeta potential was -25.4 mV which indicates the good electrostatic stability of nano-liposomes. Also, a slight size distribution (PDI 0.2870) and a high encapsulation efficiency (EE% >82% and DL>28%) are other features of synthesized nano-liposomal loaded Genipin. The in vitro result profile demonstrated that the drug-controlled release from Genipin loaded-liposomal is 65% during 70h. The in vitro cytotoxic activity of nano-liposomal loaded Genipin in comparison with free Genipin, was explored on MCF-7 cell line using MTT colorimetric assay. Our results revealed that the IC50% (cytotoxicity) of MCF-7 cells treated with nano-liposomes loaded Genipin were higher than those treated with free Genipin (about 2.4 orders of magnitude). Additionally, cell uptake studies evidenced a higher uptake of negative nano-liposomal loaded Genipin. Conclusion: In a nutshell, newly formulated nano-liposomal is an ideal vehicle for negative targeting (anticancer effect) of drugs to tumor cells that may result in improved efficacy and reduced toxicity of encapsulated drug moiety. |
| Databáze: | Directory of Open Access Journals |
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