| Popis: |
In recent years, ecotoxicological studies have been conducted around the world, especially in aquatic ecosystems. It is noteworthy that genotoxicity tests have been frequently used in the biomonitoring of pollution. The present study evaluates genotoxicity tests including comet assays, micronucleus assays, fluorescent in-situ hybridization assays, chromosomal aberration assays, and sister chromatid exchange assays used in fish species for biomonitoring of environmental pollution in Turkey. Thirty-six ecotoxicological studies, which aimed to investigate possible genotoxic damage in fish species between the years 1970 and 2016, were analyzed. As a result of the literature review, it was concluded that the largest number of studies were done in 2011. There were micronucleus assays in 83.33%, comet assays in 30.55%, chromosomal aberration assays in 5.55%, and sister chromatid exchange experiments in 2.77% of the studies. Most of the micronucleus experiments were performed in eco-genotoxicological studies. Our results show that there were no statistically significant differences (p>0.05) between the erythrocyte and gill samples in the micronucleus results from the studies. This study is the first assessment of the use of genotoxicity tests in ecotoxicological studies in Turkey. Considering the principle of refinement in 4 R theory our results show that the use of gill cells instead of peripheral erythrocytes would be more appropriate in micronucleus assays. |
