Inhibition of Pasteurella multocida Adhesion to Rabbit Respiratory Epithelium Using Lectins
| Autor: | Magda Patricia Carrillo, Nhora María Martinez, María del Pilar Patiño, Carlos Arturo Iregui |
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| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: | |
| Zdroj: | Veterinary Medicine International, Vol 2015 (2015) |
| Druh dokumentu: | article |
| ISSN: | 2090-8113 2042-0048 |
| DOI: | 10.1155/2015/365428 |
| Popis: | This study aimed to evaluate the ability of a panel of lectins to inhibit the ability of Pasteurella multocida to adhere to and affect the rabbit respiratory epithelium. Nasal septa from rabbit fetuses were cultured with various lectins before the addition of P. multocida. The percentage of bacteria adhering to the epithelium was evaluated semiquantitatively by indirect immunoperoxidase (IIP) staining. The goblet cells (GCs) were counted in semithin sections stained with toluidine blue and served as the main morphological criterion to evaluate the inhibitory effect of the lectins. The lectins PNA, WGA, RCA120, and DBA significantly inhibited the adhesion of P. multocida to the ciliated epithelium P |
| Databáze: | Directory of Open Access Journals |
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