Biodegradable Chitosan Sutures Enhanced with N-Acetyl-D-Glucosamine: Comparative Study with Catgut Sutures
| Autor: | Milena Costa da Silva Barbosa, Henrique Nunes da Silva, Débora de Sousa Lopes, Wvandson Felipe Wanderley, Rosana Araújo Rosendo, Elizandra Silva da Penha, Luanna Abílio Diniz Melquíades de Medeiros, Suédina Maria de Lima Silva, Marcus Vinícius Lia Fook |
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| Jazyk: | angličtina |
| Rok vydání: | 2024 |
| Předmět: | |
| Zdroj: | Materials Research, Vol 27 (2024) |
| Druh dokumentu: | article |
| ISSN: | 1516-1439 1980-5373 |
| DOI: | 10.1590/1980-5373-mr-2024-0278 |
| Popis: | This study aimed to develop and evaluate chitosan (CS) and chitosan with N-Acetyl-D-glucosamine (CS/GlcNAc) sutures, comparing them to commercial Catgut sutures. Medium molecular weight chitosan was processed into sutures using the wet-spinning method, both with and without the addition of GlcNAc. The mechanical properties, surface morphology, biocompatibility, biodegradability and in vivo response of the sutures were evaluated. Scanning electron microscopy (SEM) and enzymatic degradation tests in phosphate-buffered saline (PBS) with lysozyme were conducted. Cytotoxicity was assessed using an agar diffusion assay on L929 cells. In vivo studies involved suturing rat skin, with monitoring at 5, 15 and 25 days post-surgery. Both macroscopic and microscopic analyses were performed to evaluate wound healing, tissue response, and complications. The chitosan-based sutures exhibited tensile strength within the standards for surgical sutures under dry and hydrated conditions. Particularly, the CS/GlcNAc sutures exhibited superior biodegradability, and biocompatibility compared to Catgut sutures. These sutures promoted tissue healing, reduced adverse tissue reactions and infections, and displayed non-toxic to L929 cells and favorable microbiological characteristics. Thus, CS and CS/GlcNAc sutures present promising alternatives to traditional Catgut sutures, contributing valuable advancements to the field of biodegradable surgical materials. |
| Databáze: | Directory of Open Access Journals |
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