Optimizing Two-Color Semiconductor Nanocrystal Immunoassays in Single Well Microtiter Plate Formats

Autor: W. Russ Algar, Igor L. Medintz, Guoliang Tao, Robert C. Triulzi, Samantha Spindel, Travis Jennings, Kim E. Sapsford
Jazyk: angličtina
Rok vydání: 2011
Předmět:
Zdroj: Sensors, Vol 11, Iss 8, Pp 7879-7891 (2011)
Druh dokumentu: article
ISSN: 11080787
1424-8220
DOI: 10.3390/s110807879
Popis: The simultaneous detection of two analytes, chicken IgY (IgG) and Staphylococcal enterotoxin B (SEB), in the single well of a 96-well plate is demonstrated using luminescent semiconductor quantum dot nanocrystal (NC) tracers. The NC-labeled antibodies were prepared via sulfhydryl-reactive chemistry using a facile protocol that took
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